고려대 바이오유체시스템연구실

RESEARCH

1. Pressure and immisciblity based cell free DNA extraction system

● Research of sample pretreatment original technology for detection of cell free DNA ● Disposable kit design for low cost, high yield gene extraction without centrifuge ● Extraction of cfDNA using pressure gradient and non-polar solvent ● Development of integrated system of preprocessing and detection unit aiming at sample to answer system
2. DNA amplification molecular diagnostic kit based on microfluidic system

● Development of nucleic acid amplification kit using microchannel micropump ● Development of internal micro pump for closed loop circulation system ● User easily can control the circulation time according to DNA length ● PCR chip can be developed as μTAS (micro total analysis system) of microfluidic system
3. Development of virus diagnostic microchip technology for DNA detection

● Real-time detection of virus based on iso-amplification technology using microbead ● Target nucleic acid amplification channel is clogging in microbead pore ● High sensitivity based on molecular diagnostics ● Fast, multiple diagnostic tests (less than 15 minutes)
4. Hemorheologic characteristics of red blood cells based on microfluidics

● Analysis of hemorheologic characteristics of red blood cells by shear flow - Low shear = RBC aggragation, high shear = RBC deformability ● Development of hemorrhagic factor measurement technology in micro channel (Rheoscan) ● Clinical validity according to changes in red blood cells cohesion, deformability and CSS characteristics
5. Platelet activation and platelet inspection by shear flow

● Study on basic mechanism of platelet inspection using SIPA ● High shear flow generation and internal flow analysis through speed rotation of stirrer ● Establishment of optimal platelet activation condition and analysis of platelet aggregation type ● optimal microfiltration conditions by platelet activation and aggregation
6. Development of fungi separation and crushing technology based on microfluidics

● Development of environmental nanosensor system for real-time analysis of fungi in living air ● Development of pre-treatment technology for fungi separation and disruption using microfluidic system ● Selection candidate for integration with FET & peptide-based sensor ● Securing original technology of pre-tretment of mold and analysis of separation and crushing princlple

PUBLICATIONS

An innovative charge-based extracellular vesicle isolation method for highly efficient extracti…

Journal of Extracellular Biology

Junsoo Park, Minju Bae, Hyeonah Seong, Jin hwa Hong, Su Jin Kang, Kyung hwa Park, Sehyun Shin

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Rapid microfluidic-thromboelastography (μ-TEG) for evaluating whole blood coagulation and fibr…

Sensors and Actuators B: Chemical

Jikang Wang, Cheol Ung Choi, Sehyun Shin

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Collection and detection of SARS-CoV-2 in exhaled breath using face mask

Plus One

Hwang-soo Kim, Hansol Lee, Junsoo Park, Naseem Abbas, Seonghui Kang, Hakjun Hyun, Hye Seong, Jin Gu Yoon, Ji Yun Noh, Woo Joo Kim , Sehyun Shin

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